@article{geisler2026targeted,title={Preparation of targeted lipid nanoparticles for precision nucleic acid delivery},author={Geisler, Hannah C. and Battistini, Elisa and Thatte, Ajay S. and Padilla, Marshall S. and Mitchell, Michael J.},journal={Nature Protocols},year={2026},doi={10.1038/s41596-025-01330-w}}
2025
Nat. Commun.
Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering
Marshall S. Padilla, Kaitlin Mrksich, Yiming Wang, and 18 more authors
@article{padilla2025bend,title={Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering},author={Padilla, Marshall S. and Mrksich, Kaitlin and Wang, Yiming and Haley, Rebecca M. and Li, Jacqueline J. and Han, Emily L. and El-Mayta, Rakan and Kim, Emily H. and Dias, Sofia and Gong, Ningqiang and Teerdhala, Sridatta V. and Han, Xuexiang and Chowdhary, Vivek and Xue, Lulu and Siddiqui, Zain and Yamagata, Hannah M. and Kim, Dongyoon and Yoon, Il-Chul and Wilson, James M. and Radhakrishnan, Ravi and Mitchell, Michael J.},journal={Nature Communications},volume={16},year={2025},doi={10.1038/s41467-024-55137-6},}
Nat. Biotechnol.
Elucidating lipid nanoparticle properties and structure through biophysical analyses
Marshall S. Padilla, Sarah J. Shepherd, Andrew R. Hanna, and 11 more authors
@article{padilla2025biophysical,title={Elucidating lipid nanoparticle properties and structure through biophysical analyses},author={Padilla, Marshall S. and Shepherd, Sarah J. and Hanna, Andrew R. and Kurnik, Martin and Zhang, Xujun and Chen, Michelle and Byrnes, James and Joseph, Ryann A. and Yamagata, Hannah M. and Ricciardi, Adele S. and Mrksich, Kaitlin and Issadore, David and Gupta, Kushol and Mitchell, Michael J.},journal={Nature Biotechnology},year={2025},doi={10.1038/s41587-025-02855-x},}
Nano Lett.
Cas9 protein outperforms mRNA in lipid nanoparticle-mediated CFTR repair
Ryann A. Joseph, Rebecca M. Haley, Marshall S. Padilla, and 3 more authors
@article{joseph2025cas9,title={Cas9 protein outperforms mRNA in lipid nanoparticle-mediated CFTR repair},author={Joseph, Ryann A. and Haley, Rebecca M. and Padilla, Marshall S. and Ricciardi, Adele S. and Yamagata, Hannah M. and Mitchell, Michael J.},journal={Nano Letters},volume={25},number={39},pages={14348--14355},year={2025},doi={10.1021/acs.nanolett.5c03548}}
Nano Lett.
Peptide-functionalized lipid nanoparticles for targeted systemic mRNA delivery to the brain
Emily L. Han, Sophia Tang, Dongyoon Kim, and 8 more authors
@article{han2025brain,title={Peptide-functionalized lipid nanoparticles for targeted systemic mRNA delivery to the brain},author={Han, Emily L. and Tang, Sophia and Kim, Dongyoon and Murray, Amanda M. and Swingle, Kelsey L. and Hamilton, Alex G. and Mrksich, Kaitlin and Padilla, Marshall S. and Palanki, Rohan and Li, Jacqueline J. and Mitchell, Michael J.},journal={Nano Letters},year={2025},doi={10.1021/acs.nanolett.4c05186}}
2024
Adv. Drug Deliv. Rev.
Breaking the final barrier: evolution of cationic and ionizable lipid structure in lipid nanoparticles to escape the endosome
Kaitlin Mrksich, Marshall S. Padilla, and Michael J. Mitchell
@article{mrksich2024endosome,title={Breaking the final barrier: evolution of cationic and ionizable lipid structure in lipid nanoparticles to escape the endosome},author={Mrksich, Kaitlin and Padilla, Marshall S. and Mitchell, Michael J.},journal={Advanced Drug Delivery Reviews},volume={214},pages={115446},year={2024},doi={10.1016/j.addr.2024.115446},}