publications

2026

1. Chem

   

   Review

   Synthesis and optimization of ionizable lipids for RNA delivery

   Bowen Wu, Xuehe Lu, Marshall S. Padilla, Ether Dharmesh, Ying Xu, Michael J. Mitchell, and Xuexiang Han

   Chem, 2026

   @article{wu2026ionizable,
     article_type = {Review},
     title = {Synthesis and optimization of ionizable lipids for RNA delivery},
     author = {Wu, Bowen and Lu, Xuehe and Padilla, Marshall S. and Dharmesh, Ether and Xu, Ying and Mitchell, Michael J. and Han, Xuexiang},
     journal = {Chem},
     pages = {103244},
     year = {2026},
     doi = {10.1016/j.chempr.2026.103244}
   }

2. Nat. Mater.

   

   News and Views

   A boost for CAR T cell therapy

   Emily Fitzgerald, Marshall S. Padilla, and Michael J. Mitchell

   Nature Materials, 2026

   @article{fitzgerald2026cart,
     article_type = {News and Views},
     title = {A boost for CAR T cell therapy},
     author = {Fitzgerald, Emily and Padilla, Marshall S. and Mitchell, Michael J.},
     journal = {Nature Materials},
     year = {2026},
     doi = {10.1038/s41563-026-02725-0}
   }

3. Adv. Mater.

   

   Research Article

   Lipid nanoparticle co-delivery of mRNA and a small molecule drug for oral cancer chemoimmunotherapy

   Marshall S. Padilla, Jacqueline J. Li, Qunzhou Zhang, Korey Patwari, Shihong Shi, Hannah M. Yamagata, Ryann A. Joseph, Briyanna N. Hymms, Sridatta V. Teerdhala, Mohamad-Gabriel Alameh, Drew Weissman, Anh D. Le, and Michael J. Mitchell

   Advanced Materials, 2026

   @article{padilla2026oral,
     article_type = {Research Article},
     title = {Lipid nanoparticle co-delivery of mRNA and a small molecule drug for oral cancer chemoimmunotherapy},
     author = {Padilla, Marshall S. and Li, Jacqueline J. and Zhang, Qunzhou and Patwari, Korey and Shi, Shihong and Yamagata, Hannah M. and Joseph, Ryann A. and Hymms, Briyanna N. and Teerdhala, Sridatta V. and Alameh, Mohamad-Gabriel and Weissman, Drew and Le, Anh D. and Mitchell, Michael J.},
     journal = {Advanced Materials},
     year = {2026},
     doi = {10.1002/adma.73721}
   }

4. Nano Lett.

   

   Research Article

   Enhancing lipid nanoparticle-mediated circular RNA and mRNA expression in the placenta through inhibition of IFNAR-JAK-STAT signaling

   Hannah C. Safford, Hannah C. Geisler, Ajay S. Thatte, Kuo-Chieh. Liao, Marshall S. Padilla, Hannah M. Yamagata, Amanda M. Murray, Anushka. Agrawal, Alexandre. Poirier, Alex G. Hamilton, Kelsey L. Swingle, Emily. Fitzgerald, Ori Chalom, Yue Wan, and Michael J. Mitchell

   Nano Letters, 2026

   @article{safford2026circrna,
     article_type = {Research Article},
     title = {Enhancing lipid nanoparticle-mediated circular RNA and mRNA expression in the placenta through inhibition of IFNAR-JAK-STAT signaling},
     author = {Safford, Hannah C. and Geisler, Hannah C. and Thatte, Ajay S. and Liao, Kuo-Chieh. and Padilla, Marshall S. and Yamagata, Hannah M. and Murray, Amanda M. and Agrawal, Anushka. and Poirier, Alexandre. and Hamilton, Alex G. and Swingle, Kelsey L. and Fitzgerald, Emily. and Chalom, Ori and Wan, Yue and Mitchell, Michael J.},
     journal = {Nano Letters},
     year = {2026},
     doi = {10.1021/acs.nanolett.6c01762}
   }

5. Adv. Mater.

   

   Research Article

   Resolving heterogeneity of targeted lipid nanoparticles through solution-based biophysical analyses

   Hannah C. Geisler, Hannah C. Safford, Ajay S. Thatte, Marshall S. Padilla, Elisa Battistini, Hannah M. Yamagata, Violet M. Ullman, Alex Chan, Benjamin E. Nachod, Anushka Agrawal, Maxwell B. Watkins, Jesse B. Hopkins, Andrew Tsourkas, Kushol Gupta, and Michael J. Mitchell

   Advanced Materials, 2026

   @article{geisler2026tlnp,
     article_type = {Research Article},
     title = {Resolving heterogeneity of targeted lipid nanoparticles through solution-based biophysical analyses},
     author = {Geisler, Hannah C. and Safford, Hannah C. and Thatte, Ajay S. and Padilla, Marshall S. and Battistini, Elisa and Yamagata, Hannah M. and Ullman, Violet M. and Chan, Alex and Nachod, Benjamin E. and Agrawal, Anushka and Watkins, Maxwell B. and Hopkins, Jesse B. and Tsourkas, Andrew and Gupta, Kushol and Mitchell, Michael J.},
     journal = {Advanced Materials},
     year = {2026},
     doi = {10.1002/adma.73984}
   }

6. PNAS

   

   Research Article

   mRNA lipid nanoparticle cancer vaccine platform delivering multiple STING activators for enhanced antitumor activity

   Ye Zeng, Junchao Xu, Jinjin Wang, Lulu Xue, Jiageng Liu, Hannah C. Geisler, Xu Ma, Jilian R. Melamed, Qiangqiang Shi, Marshall S. Padilla, Zhangyi Luo, Jingcheng Zhu, Ajay S. Thatte, Christian G. Figueroa-Espada, Melgious Jin Yan Ang, Amanda M. Murray, Hannah M. Yamagata, Dongyoon Kim, Ann E. Metzloff, Drew Weissman, and Michael J. Mitchell

   Proceedings of the National Academy of Sciences, 2026

   @article{zeng2026sting,
     article_type = {Research Article},
     title = {mRNA lipid nanoparticle cancer vaccine platform delivering multiple STING activators for enhanced antitumor activity},
     author = {Zeng, Ye and Xu, Junchao and Wang, Jinjin and Xue, Lulu and Liu, Jiageng and Geisler, Hannah C. and Ma, Xu and Melamed, Jilian R. and Shi, Qiangqiang and Padilla, Marshall S. and Luo, Zhangyi and Zhu, Jingcheng and Thatte, Ajay S. and Figueroa-Espada, Christian G. and Ang, Melgious Jin Yan and Murray, Amanda M. and Yamagata, Hannah M. and Kim, Dongyoon and Metzloff, Ann E. and Weissman, Drew and Mitchell, Michael J.},
     journal = {Proceedings of the National Academy of Sciences},
     volume = {123},
     number = {28},
     pages = {e2525718123},
     year = {2026},
     doi = {10.1073/pnas.2525718123}
   }

7. Nat. Protoc.

   

   Protocol

   Preparation of targeted lipid nanoparticles for precision nucleic acid delivery

   Hannah C. Geisler, Elisa Battistini, Ajay S. Thatte, Marshall S. Padilla, and Michael J. Mitchell

   Nature Protocols, 2026

   In press

   @article{geisler2026protocols,
     article_type = {Protocol},
     title = {Preparation of targeted lipid nanoparticles for precision nucleic acid delivery},
     author = {Geisler, Hannah C. and Battistini, Elisa and Thatte, Ajay S. and Padilla, Marshall S. and Mitchell, Michael J.},
     journal = {Nature Protocols},
     note = {In press},
     year = {2026},
     doi = {10.1038/s41596-025-01330-w}
   }

8. JACS

   

   Research Article

   Aromatic mRNA lipid nanoparticle (aroLNP) platform confers lymph node tropism and vaccine protection with reduced liver delivery

   Hannah M. Yamagata, Marshall S. Padilla, Alex G. Hamilton, Kelsey L. Swingle, Adele S. Ricciardi, Ajay S. Thatte, Ryann A. Joseph, Ori Chalom, Hannah C. Geisler, Jacqueline J. Li, Akash Agrawal, Sherry Du, Dongyoon Kim, Rachel Whitaker, Christian G. Figueroa-Espada, Emily L. Han, Amanda M. Murray, Mohamad-Gabriel Alameh, Drew Weissman, and Michael J. Mitchell

   Journal of the American Chemical Society, 2026

   @article{yamagata2026arolnp,
     article_type = {Research Article},
     title = {Aromatic mRNA lipid nanoparticle (aroLNP) platform confers lymph node tropism and vaccine protection with reduced liver delivery},
     author = {Yamagata, Hannah M. and Padilla, Marshall S. and Hamilton, Alex G. and Swingle, Kelsey L. and Ricciardi, Adele S. and Thatte, Ajay S. and Joseph, Ryann A. and Chalom, Ori and Geisler, Hannah C. and Li, Jacqueline J. and Agrawal, Akash and Du, Sherry and Kim, Dongyoon and Whitaker, Rachel and Figueroa-Espada, Christian G. and Han, Emily L. and Murray, Amanda M. and Alameh, Mohamad-Gabriel and Weissman, Drew and Mitchell, Michael J.},
     journal = {Journal of the American Chemical Society},
     volume = {148},
     pages = {14137--14150},
     year = {2026},
     doi = {10.1021/jacs.6c00080}
   }

9. ACS Nano

   

   Research Article

   Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries

   Andrew R. Hanna, Sarah J. Shepherd, Gregory A. Datto, Ian Navarro, Adele S. Ricciardi, Marshall S. Padilla, Neha Srikumar, Sheng Zhang, Hannah M. Yamagata, Nina Y. Meng, Joshua R. Buser, Michael J. Mitchell, and David Issadore

   ACS Nano, 2026

   @article{hanna2026microfluidic,
     article_type = {Research Article},
     title = {Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries},
     author = {Hanna, Andrew R. and Shepherd, Sarah J. and Datto, Gregory A. and Navarro, Ian and Ricciardi, Adele S. and Padilla, Marshall S. and Srikumar, Neha and Zhang, Sheng and Yamagata, Hannah M. and Meng, Nina Y. and Buser, Joshua R. and Mitchell, Michael J. and Issadore, David},
     journal = {ACS Nano},
     volume = {20},
     pages = {772--789},
     year = {2026},
     doi = {10.1021/acsnano.5c15613}
   }

2025

1. Nat. Commun.

   

   Research Article

   Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering

   Marshall S. Padilla, Kaitlin Mrksich, Yiming Wang, Rebecca M. Haley, Jacqueline J. Li, Emily L. Han, Rakan El-Mayta, Emily H. Kim, Sofia Dias, Ningqiang Gong, Sridatta V. Teerdhala, Xuexiang Han, Vivek Chowdhary, Lulu Xue, Zain Siddiqui, Hannah M. Yamagata, Dongyoon Kim, Il-Chul Yoon, James M. Wilson, Ravi Radhakrishnan, and Michael J. Mitchell

   Nature Communications, 2025

   @article{padilla2025bend,
     article_type = {Research Article},
     title = {Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering},
     author = {Padilla, Marshall S. and Mrksich, Kaitlin and Wang, Yiming and Haley, Rebecca M. and Li, Jacqueline J. and Han, Emily L. and El-Mayta, Rakan and Kim, Emily H. and Dias, Sofia and Gong, Ningqiang and Teerdhala, Sridatta V. and Han, Xuexiang and Chowdhary, Vivek and Xue, Lulu and Siddiqui, Zain and Yamagata, Hannah M. and Kim, Dongyoon and Yoon, Il-Chul and Wilson, James M. and Radhakrishnan, Ravi and Mitchell, Michael J.},
     journal = {Nature Communications},
     volume = {16},
     pages = {996},
     year = {2025},
     doi = {10.1038/s41467-024-55137-6}
   }

2. Nat. Biotechnol.

   

   Research Article

   Elucidating lipid nanoparticle properties and structure through biophysical analyses

   Marshall S. Padilla, Sarah J. Shepherd, Andrew R. Hanna, Martin Kurnik, Xujun Zhang, Michelle Chen, James Byrnes, Ryann A. Joseph, Hannah M. Yamagata, Adele S. Ricciardi, Kaitlin Mrksich, David Issadore, Kushol Gupta, and Michael J. Mitchell

   Nature Biotechnology, 2025

   In press

   @article{padilla2025biophysical,
     article_type = {Research Article},
     title = {Elucidating lipid nanoparticle properties and structure through biophysical analyses},
     author = {Padilla, Marshall S. and Shepherd, Sarah J. and Hanna, Andrew R. and Kurnik, Martin and Zhang, Xujun and Chen, Michelle and Byrnes, James and Joseph, Ryann A. and Yamagata, Hannah M. and Ricciardi, Adele S. and Mrksich, Kaitlin and Issadore, David and Gupta, Kushol and Mitchell, Michael J.},
     journal = {Nature Biotechnology},
     note = {In press},
     year = {2025},
     doi = {10.1038/s41587-025-02855-x}
   }

3. Nat. Biotechnol.

   

   Research Briefing

   Solution biophysics reveals the polydisperse structure of RNA lipid nanoparticles

   Marshall S. Padilla, Kushol Gupta, and Michael J. Mitchell

   Nature Biotechnology, 2025

   In press

   @article{padilla2025solutionbiophysics,
     article_type = {Research Briefing},
     title = {Solution biophysics reveals the polydisperse structure of RNA lipid nanoparticles},
     author = {Padilla, Marshall S. and Gupta, Kushol and Mitchell, Michael J.},
     journal = {Nature Biotechnology},
     note = {In press},
     year = {2025},
     doi = {10.1038/s41587-025-02909-0}
   }

4. Nano Lett.

   

   Research Article

   Cas9 protein enhances lipid nanoparticle-mediated CFTR repair compared to mRNA

   Ryann A. Joseph, Rebecca M. Haley, Marshall S. Padilla, Adele S. Ricciardi, Hannah M. Yamagata, and Michael J. Mitchell

   Nano Letters, 2025

   @article{joseph2025cas9,
     article_type = {Research Article},
     title = {Cas9 protein enhances lipid nanoparticle-mediated CFTR repair compared to mRNA},
     author = {Joseph, Ryann A. and Haley, Rebecca M. and Padilla, Marshall S. and Ricciardi, Adele S. and Yamagata, Hannah M. and Mitchell, Michael J.},
     journal = {Nano Letters},
     volume = {25},
     pages = {14348--14355},
     year = {2025},
     doi = {10.1021/acs.nanolett.5c03548}
   }

5. ACS Nano

   

   Research Article

   Lipid nanoparticles for in vivo lung delivery of CRISPR-Cas9 ribonucleoproteins allow gene editing of clinical targets

   Rebecca M. Haley, Marshall S. Padilla, Rakan D. El-Mayta, Ryann A. Joseph, Jamie A. Weber, Christian G. Figueroa-Espada, Alvin J. Mukalel, Adele S. Ricciardi, Rohan Palanki, Hannah C. Geisler, Margaret T. Jester, Beverly L. Davidson, and Michael J. Mitchell

   ACS Nano, 2025

   @article{haley2025lung,
     article_type = {Research Article},
     title = {Lipid nanoparticles for in vivo lung delivery of CRISPR-Cas9 ribonucleoproteins allow gene editing of clinical targets},
     author = {Haley, Rebecca M. and Padilla, Marshall S. and El-Mayta, Rakan D. and Joseph, Ryann A. and Weber, Jamie A. and Figueroa-Espada, Christian G. and Mukalel, Alvin J. and Ricciardi, Adele S. and Palanki, Rohan and Geisler, Hannah C. and Jester, Margaret T. and Davidson, Beverly L. and Mitchell, Michael J.},
     journal = {ACS Nano},
     volume = {19},
     pages = {13790--13804},
     year = {2025},
     doi = {10.1021/acsnano.4c16617}
   }

2024

1. Eur. J. Immunol.

   

   Review

   Lipid nanoparticle-mediated RNA delivery for immune cell modulation

   Emily H.* Kim, Sridatta V.* Teerdhala, Marshall S.* Padilla, Ryann A. Joseph, Jacqueline J. Li, Rebecca M. Haley, and Michael J. Mitchell

   European Journal of Immunology, 2024

   @article{kim2024immune,
     article_type = {Review},
     title = {Lipid nanoparticle-mediated RNA delivery for immune cell modulation},
     author = {Kim, Emily H. and Teerdhala, Sridatta V. and Padilla, Marshall S. and Joseph, Ryann A. and Li, Jacqueline J. and Haley, Rebecca M. and Mitchell, Michael J.},
     journal = {European Journal of Immunology},
     volume = {54},
     pages = {2451008},
     year = {2024},
     doi = {10.1002/eji.202451008}
   }

2. Adv. Drug Deliv. Rev.

   

   Review

   Breaking the final barrier: evolution of cationic and ionizable lipid structure in lipid nanoparticles to escape the endosome

   Kaitlin* Mrksich, Marshall S.* Padilla, and Michael J. Mitchell

   Advanced Drug Delivery Reviews, 2024

   @article{mrksich2024endosome,
     article_type = {Review},
     title = {Breaking the final barrier: evolution of cationic and ionizable lipid structure in lipid nanoparticles to escape the endosome},
     author = {Mrksich, Kaitlin and Padilla, Marshall S. and Mitchell, Michael J.},
     journal = {Advanced Drug Delivery Reviews},
     volume = {214},
     pages = {115446},
     year = {2024},
     doi = {10.1016/j.addr.2024.115446}
   }

3. J. Mol. Liq.

   

   Review

   Probing ionic liquid toxicity through biophysical and computational methods

   Marshall S. Padilla and Sandro Mecozzi

   Journal of Molecular Liquids, 2024

   @article{padilla2024ionicliquid,
     article_type = {Review},
     title = {Probing ionic liquid toxicity through biophysical and computational methods},
     author = {Padilla, Marshall S. and Mecozzi, Sandro},
     journal = {Journal of Molecular Liquids},
     volume = {395},
     pages = {123886},
     year = {2024},
     doi = {10.1016/j.molliq.2023.123886}
   }

4. Nano Lett.

   

   Research Article

   Peptide-functionalized lipid nanoparticles for targeted systemic mRNA delivery to the brain

   Emily L. Han, Sophia Tang, Dongyoon Kim, Amanda M. Murray, Kelsey L. Swingle, Alex G. Hamilton, Kaitlin Mrksich, Marshall S. Padilla, Rohan Palanki, Jacqueline J. Li, and Michael J. Mitchell

   Nano Letters, 2024

   @article{han2024brain,
     article_type = {Research Article},
     title = {Peptide-functionalized lipid nanoparticles for targeted systemic mRNA delivery to the brain},
     author = {Han, Emily L. and Tang, Sophia and Kim, Dongyoon and Murray, Amanda M. and Swingle, Kelsey L. and Hamilton, Alex G. and Mrksich, Kaitlin and Padilla, Marshall S. and Palanki, Rohan and Li, Jacqueline J. and Mitchell, Michael J.},
     journal = {Nano Letters},
     volume = {25},
     pages = {800--810},
     year = {2024},
     doi = {10.1021/acs.nanolett.4c05186}
   }

5. Nat. Chem.

   

   Research Article

   Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo

   Xuexiang Han, Mohamad-Gabriel Alameh, Ningqiang Gong, Lulu Xue, Majed Ghattas, Giridhar Bojja, Junchao Xu, Gan Zhao, Claude C. Warzecha, Marshall S. Padilla, Rakan El-Mayta, Garima Dwivedi, Yiming Xu, Andrew E. Vaughan, James M. Wilson, Drew Weissman, and Michael J. Mitchell

   Nature Chemistry, 2024

   @article{han2024amidine,
     article_type = {Research Article},
     title = {Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo},
     author = {Han, Xuexiang and Alameh, Mohamad-Gabriel and Gong, Ningqiang and Xue, Lulu and Ghattas, Majed and Bojja, Giridhar and Xu, Junchao and Zhao, Gan and Warzecha, Claude C. and Padilla, Marshall S. and El-Mayta, Rakan and Dwivedi, Garima and Xu, Yiming and Vaughan, Andrew E. and Wilson, James M. and Weissman, Drew and Mitchell, Michael J.},
     journal = {Nature Chemistry},
     volume = {16},
     pages = {1687--1697},
     year = {2024},
     doi = {10.1038/s41557-024-01557-2}
   }

6. Nano Lett.

   

   Research Article

   Predictive high-throughput platform for dual screening of mRNA lipid nanoparticle blood-brain barrier transfection and crossing

   Emily L. Han, Marshall S. Padilla, Rohan Palanki, Dongyoon Kim, Kaitlin Mrksich, Jacqueline J. Li, Sophia Tang, Il-Chul Yoon, and Michael J. Mitchell

   Nano Letters, 2024

   @article{han2024bbb,
     article_type = {Research Article},
     title = {Predictive high-throughput platform for dual screening of mRNA lipid nanoparticle blood-brain barrier transfection and crossing},
     author = {Han, Emily L. and Padilla, Marshall S. and Palanki, Rohan and Kim, Dongyoon and Mrksich, Kaitlin and Li, Jacqueline J. and Tang, Sophia and Yoon, Il-Chul and Mitchell, Michael J.},
     journal = {Nano Letters},
     volume = {24},
     pages = {1477--1486},
     year = {2024},
     doi = {10.1021/acs.nanolett.3c03509}
   }

7. Adv. Mater.

   

   Research Article

   Antigen presenting cell mimetic lipid nanoparticles for rapid mRNA CAR T cell cancer immunotherapy

   Ann E. Metzloff, Marshall S. Padilla, Ningqiang Gong, Margaret M. Billingsley, Xuexiang Han, Maria Merolle, Dan Mai, Christian G. Figueroa-Espada, Ajay S. Thatte, Rebecca M. Haley, Alvin J. Mukalel, Alex G. Hamilton, Mohamad-Gabriel Alameh, Drew Weissman, Neil C. Sheppard, Carl H. June, and Michael J. Mitchell

   Advanced Materials, 2024

   @article{metzloff2024apc,
     article_type = {Research Article},
     title = {Antigen presenting cell mimetic lipid nanoparticles for rapid mRNA CAR T cell cancer immunotherapy},
     author = {Metzloff, Ann E. and Padilla, Marshall S. and Gong, Ningqiang and Billingsley, Margaret M. and Han, Xuexiang and Merolle, Maria and Mai, Dan and Figueroa-Espada, Christian G. and Thatte, Ajay S. and Haley, Rebecca M. and Mukalel, Alvin J. and Hamilton, Alex G. and Alameh, Mohamad-Gabriel and Weissman, Drew and Sheppard, Neil C. and June, Carl H. and Mitchell, Michael J.},
     journal = {Advanced Materials},
     volume = {36},
     pages = {2313226},
     year = {2024},
     doi = {10.1002/adma.202313226}
   }

8. J. Biomed. Mater. Res. A

   

   Research Article

   Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length

   Kaitlin Mrksich, Marshall S. Padilla, Ryann A. Joseph, Emily L. Han, Dongyoon Kim, Rohan Palanki, Junchao Xu, and Michael J. Mitchell

   Journal of Biomedical Materials Research Part A, 2024

   @article{mrksich2024taillength,
     article_type = {Research Article},
     title = {Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length},
     author = {Mrksich, Kaitlin and Padilla, Marshall S. and Joseph, Ryann A. and Han, Emily L. and Kim, Dongyoon and Palanki, Rohan and Xu, Junchao and Mitchell, Michael J.},
     journal = {Journal of Biomedical Materials Research Part A},
     volume = {112},
     pages = {1494--1505},
     year = {2024},
     doi = {10.1002/jbm.a.37705}
   }

9. J. Control. Release

   

   Research Article

   EGFR-targeted ionizable lipid nanoparticles enhance in vivo mRNA delivery to the placenta

   Hannah C. Geisler, Aditya A. Ghalsasi, Hannah C. Safford, Kelsey L. Swingle, Ajay S. Thatte, Alvin J. Mukalel, Ningqiang Gong, Alex G. Hamilton, Emily L. Han, Benjamin E. Nachod, Marshall S. Padilla, and Michael J. Mitchell

   Journal of Controlled Release, 2024

   @article{geisler2024egfr,
     article_type = {Research Article},
     title = {EGFR-targeted ionizable lipid nanoparticles enhance in vivo mRNA delivery to the placenta},
     author = {Geisler, Hannah C. and Ghalsasi, Aditya A. and Safford, Hannah C. and Swingle, Kelsey L. and Thatte, Ajay S. and Mukalel, Alvin J. and Gong, Ningqiang and Hamilton, Alex G. and Han, Emily L. and Nachod, Benjamin E. and Padilla, Marshall S. and Mitchell, Michael J.},
     journal = {Journal of Controlled Release},
     volume = {371},
     pages = {455--469},
     year = {2024},
     doi = {10.1016/j.jconrel.2024.05.036}
   }

10. Adv. Funct. Mater.

    

    Research Article

    Oxidized mRNA lipid nanoparticles for in situ chimeric antigen receptor monocyte engineering

    Alvin J. Mukalel, Alex G. Hamilton, Margaret M. Billingsley, Jacqueline Li, Ajay S. Thatte, Xuexiang Han, Hannah C. Safford, Marshall S. Padilla, Tyler Papp, Hamideh Parhiz, Drew Weissman, and Michael J. Mitchell

    Advanced Functional Materials, 2024

    @article{mukalel2024oxidized,
      article_type = {Research Article},
      title = {Oxidized mRNA lipid nanoparticles for in situ chimeric antigen receptor monocyte engineering},
      author = {Mukalel, Alvin J. and Hamilton, Alex G. and Billingsley, Margaret M. and Li, Jacqueline and Thatte, Ajay S. and Han, Xuexiang and Safford, Hannah C. and Padilla, Marshall S. and Papp, Tyler and Parhiz, Hamideh and Weissman, Drew and Mitchell, Michael J.},
      journal = {Advanced Functional Materials},
      volume = {34},
      pages = {2312038},
      year = {2024},
      doi = {10.1002/adfm.202312038}
    }

2023

1. J. Pharm. Sci.

   

   Research Article

   MCT nanoemulsions for the efficient delivery of siRNA

   Marshall S. Padilla, Montri Tangsangasaksri, Chia-Chih Chang, and Sandro Mecozzi

   Journal of Pharmaceutical Sciences, 2023

   @article{padilla2023mct,
     article_type = {Research Article},
     title = {MCT nanoemulsions for the efficient delivery of siRNA},
     author = {Padilla, Marshall S. and Tangsangasaksri, Montri and Chang, Chia-Chih and Mecozzi, Sandro},
     journal = {Journal of Pharmaceutical Sciences},
     volume = {113},
     pages = {764--771},
     year = {2023},
     doi = {10.1016/j.xphs.2023.11.013}
   }

2. ACS Appl. Mater. Interfaces

   

   Research Article

   Lipid nanoparticle delivery of small proteins for potent in vivo RAS inhibition

   Rebecca M. Haley, Alexander Chan, Margaret M. Billingsley, Ningqiang Gong, Marshall S. Padilla, Emily H. Kim, Hejia Wang, Dongni Yin, Kirk J. Wangensteen, Andrew Tsourkas, and Michael J. Mitchell

   ACS Applied Materials & Interfaces, 2023

   @article{haley2023ras,
     article_type = {Research Article},
     title = {Lipid nanoparticle delivery of small proteins for potent in vivo RAS inhibition},
     author = {Haley, Rebecca M. and Chan, Alexander and Billingsley, Margaret M. and Gong, Ningqiang and Padilla, Marshall S. and Kim, Emily H. and Wang, Hejia and Yin, Dongni and Wangensteen, Kirk J. and Tsourkas, Andrew and Mitchell, Michael J.},
     journal = {ACS Applied Materials \& Interfaces},
     volume = {15},
     pages = {21877--21892},
     year = {2023},
     doi = {10.1021/acsami.3c01501}
   }

3. Int. J. Nanomedicine

   

   Research Article

   Delivery of plasmid DNA by ionizable lipid nanoparticles to induce CAR expression in T cells

   Pedro H. D. M. Prazeres, Heloisa Ferreira, Pedro A. C. Costa, Walison Silva, Marco T. Alves, Marshall Padilla, Ajay Thatte, Anderson K. Santos, Anderson O. Lobo, Adriano Sabino, Helen L. Del Puerto, Michael J. Mitchell, and Pedro P. G. Guimaraes

   International Journal of Nanomedicine, 2023

   @article{prazeres2023plasmid,
     article_type = {Research Article},
     title = {Delivery of plasmid DNA by ionizable lipid nanoparticles to induce CAR expression in T cells},
     author = {Prazeres, Pedro H. D. M. and Ferreira, Heloisa and Costa, Pedro A. C. and da Silva, Walison and Alves, Marco T. and Padilla, Marshall and Thatte, Ajay and Santos, Anderson K. and Lobo, Anderson O. and Sabino, Adriano and Del Puerto, Helen L. and Mitchell, Michael J. and Guimaraes, Pedro P. G.},
     journal = {International Journal of Nanomedicine},
     volume = {18},
     pages = {5891--5904},
     year = {2023},
     doi = {10.2147/IJN.S424723}
   }

4. PNAS

   

   Research Article

   Throughput-scalable manufacturing of SARS-CoV-2 mRNA lipid nanoparticle vaccines

   Sarah J. Shepherd, Xuexiang Han, Alvin J. Mukalel, Rakan El-Mayta, Ajay S. Thatte, Jingya Wu, Marshall S. Padilla, Mohamad-Gabriel Alameh, Neha Srikumar, Daeyeon Lee, Drew Weissman, David Issadore, and Michael J. Mitchell

   Proceedings of the National Academy of Sciences, 2023

   @article{shepherd2023vaccine,
     article_type = {Research Article},
     title = {Throughput-scalable manufacturing of SARS-CoV-2 mRNA lipid nanoparticle vaccines},
     author = {Shepherd, Sarah J. and Han, Xuexiang and Mukalel, Alvin J. and El-Mayta, Rakan and Thatte, Ajay S. and Wu, Jingya and Padilla, Marshall S. and Alameh, Mohamad-Gabriel and Srikumar, Neha and Lee, Daeyeon and Weissman, Drew and Issadore, David and Mitchell, Michael J.},
     journal = {Proceedings of the National Academy of Sciences},
     volume = {120},
     pages = {e2303567120},
     year = {2023},
     doi = {10.1073/pnas.2303567120}
   }

5. JACS

   

   Research Article

   Ionizable lipid nanoparticles for in vivo mRNA delivery to the placenta during pregnancy

   Kelsey L. Swingle, Hannah C. Safford, Hannah C. Geisler, Alex G. Hamilton, Ajay S. Thatte, Margaret M. Billingsley, Ryann A. Joseph, Kaitlin Mrksich, Marshall S. Padilla, Aditya A. Ghalsasi, Mohamad-Gabriel Alameh, Drew Weissman, and Michael J. Mitchell

   Journal of the American Chemical Society, 2023

   @article{swingle2023placenta,
     article_type = {Research Article},
     title = {Ionizable lipid nanoparticles for in vivo mRNA delivery to the placenta during pregnancy},
     author = {Swingle, Kelsey L. and Safford, Hannah C. and Geisler, Hannah C. and Hamilton, Alex G. and Thatte, Ajay S. and Billingsley, Margaret M. and Joseph, Ryann A. and Mrksich, Kaitlin and Padilla, Marshall S. and Ghalsasi, Aditya A. and Alameh, Mohamad-Gabriel and Weissman, Drew and Mitchell, Michael J.},
     journal = {Journal of the American Chemical Society},
     volume = {145},
     pages = {4691--4706},
     year = {2023},
     doi = {10.1021/jacs.2c12893}
   }

6. Lab Chip

   

   Review

   Nanoparticle protein corona: from structure and function to therapeutic targeting

   Ghazal Bashiri, Marshall S. Padilla, Kelsey L. Swingle, Sarah J. Shepherd, Michael J. Mitchell, and Karen Wang

   Lab on a Chip, 2023

   @article{bashiri2023corona,
     article_type = {Review},
     title = {Nanoparticle protein corona: from structure and function to therapeutic targeting},
     author = {Bashiri, Ghazal and Padilla, Marshall S. and Swingle, Kelsey L. and Shepherd, Sarah J. and Mitchell, Michael J. and Wang, Karen},
     journal = {Lab on a Chip},
     volume = {23},
     pages = {1432--1466},
     year = {2023},
     doi = {10.1039/D2LC00799A}
   }

7. JoVE

   

   Protocol

   Testing the in vitro and in vivo efficiency of mRNA-lipid nanoparticles formulated by microfluidic mixing

   Rakan El-Mayta, Marshall S. Padilla, Margaret M. Billingsley, Xuexiang Han, and Michael J. Mitchell

   Journal of Visualized Experiments, 2023

   @article{elmayta2023jove,
     article_type = {Protocol},
     title = {Testing the in vitro and in vivo efficiency of mRNA-lipid nanoparticles formulated by microfluidic mixing},
     author = {El-Mayta, Rakan and Padilla, Marshall S. and Billingsley, Margaret M. and Han, Xuexiang and Mitchell, Michael J.},
     journal = {Journal of Visualized Experiments},
     pages = {e64810},
     year = {2023},
     doi = {10.3791/64810}
   }

2022

1. JACS

   

   Research Article

   Rational design of bisphosphonate lipid-like materials for mRNA delivery to the bone microenvironment

   Lulu Xue, Ningqiang Gong, Sarah J. Shepherd, Xinhong Xiong, Xinyu Liao, Xuexiang Han, Gan Zhao, Chunhao Song, Hanwen Zhang, Marshall S. Padilla, Jianxin Qin, Yi Shi, Mohamad-Gabriel Alameh, Darrin J. Pochan, Karen Wang, Fei Long, Drew Weissman, and Michael J. Mitchell

   Journal of the American Chemical Society, 2022

   @article{xue2022bisphosphonate,
     article_type = {Research Article},
     title = {Rational design of bisphosphonate lipid-like materials for mRNA delivery to the bone microenvironment},
     author = {Xue, Lulu and Gong, Ningqiang and Shepherd, Sarah J. and Xiong, Xinhong and Liao, Xinyu and Han, Xuexiang and Zhao, Gan and Song, Chunhao and Zhang, Hanwen and Padilla, Marshall S. and Qin, Jianxin and Shi, Yi and Alameh, Mohamad-Gabriel and Pochan, Darrin J. and Wang, Karen and Long, Fei and Weissman, Drew and Mitchell, Michael J.},
     journal = {Journal of the American Chemical Society},
     volume = {144},
     pages = {9926--9937},
     year = {2022},
     doi = {10.1021/jacs.2c02706}
   }

2. J. Biomed. Mater. Res. A

   

   Research Article

   Rational design of anti-inflammatory lipid nanoparticles for mRNA delivery

   Hanwen Zhang, Xuexiang Han, Mohamad-Gabriel Alameh, Sarah J. Shepherd, Marshall S. Padilla, Lulu Xue, Kamila Butowska, Drew Weissman, and Michael J. Mitchell

   Journal of Biomedical Materials Research Part A, 2022

   @article{zhang2022antiinflammatory,
     article_type = {Research Article},
     title = {Rational design of anti-inflammatory lipid nanoparticles for mRNA delivery},
     author = {Zhang, Hanwen and Han, Xuexiang and Alameh, Mohamad-Gabriel and Shepherd, Sarah J. and Padilla, Marshall S. and Xue, Lulu and Butowska, Kamila and Weissman, Drew and Mitchell, Michael J.},
     journal = {Journal of Biomedical Materials Research Part A},
     volume = {110},
     pages = {1101--1108},
     year = {2022},
     doi = {10.1002/jbm.a.37356}
   }

2021

1. Green Chem.

   

   Research Article

   Expanding the structural diversity of hydrophobic ionic liquids: physicochemical properties and toxicity of Gemini ionic liquids

   Marshall S. Padilla, Colin Bertz, Nicole Berdusco, and Sandro Mecozzi

   Green Chemistry, 2021

   @article{padilla2021gemini,
     article_type = {Research Article},
     title = {Expanding the structural diversity of hydrophobic ionic liquids: physicochemical properties and toxicity of Gemini ionic liquids},
     author = {Padilla, Marshall S. and Bertz, Colin and Berdusco, Nicole and Mecozzi, Sandro},
     journal = {Green Chemistry},
     volume = {23},
     pages = {4375--4385},
     year = {2021},
     doi = {10.1039/D1GC00742D}
   }

2018

1. Bioorg. Med. Chem. Lett.

   

   Research Article

   Investigation of copper-free alkyne/azide 1,3-dipolar cycloadditions using microwave irradiation

   Lindsay E. Chatkewitz, John F. Halonski, Marshall S. Padilla, and Douglas D. Young

   Bioorganic & Medicinal Chemistry Letters, 2018

   @article{chatkewitz2018microwave,
     article_type = {Research Article},
     title = {Investigation of copper-free alkyne/azide 1,3-dipolar cycloadditions using microwave irradiation},
     author = {Chatkewitz, Lindsay E. and Halonski, John F. and Padilla, Marshall S. and Young, Douglas D.},
     journal = {Bioorganic \& Medicinal Chemistry Letters},
     volume = {28},
     pages = {81--84},
     year = {2018},
     doi = {10.1016/j.bmcl.2017.12.007}
   }

2016

1. Tetrahedron Lett.

   

   Research Article

   Synthesis and incorporation of a caged tyrosine amino acid possessing a bioorthogonal handle

   Marshall S. Padilla, Connor A. Farley, Lindsay E. Chatkewitz, and Douglas D. Young

   Tetrahedron Letters, 2016

   @article{padilla2016tyrosine,
     article_type = {Research Article},
     title = {Synthesis and incorporation of a caged tyrosine amino acid possessing a bioorthogonal handle},
     author = {Padilla, Marshall S. and Farley, Connor A. and Chatkewitz, Lindsay E. and Young, Douglas D.},
     journal = {Tetrahedron Letters},
     volume = {57},
     pages = {4709--4712},
     year = {2016},
     doi = {10.1016/j.tetlet.2016.09.033}
   }

2015

1. Bioorg. Med. Chem. Lett.

   

   Research Article

   Photosensitive GFP mutants containing an azobenzene unnatural amino acid

   Marshall S. Padilla and Douglas D. Young

   Bioorganic & Medicinal Chemistry Letters, 2015

   @article{padilla2015azobenzene,
     article_type = {Research Article},
     title = {Photosensitive GFP mutants containing an azobenzene unnatural amino acid},
     author = {Padilla, Marshall S. and Young, Douglas D.},
     journal = {Bioorganic \& Medicinal Chemistry Letters},
     volume = {25},
     pages = {470--473},
     year = {2015},
     doi = {10.1016/j.bmcl.2014.12.035}
   }

2014

1. Org. Biomol. Chem.

   

   Research Article

   Preparation of asymmetrical polyynes by a solid-supported Glaser-Hay reaction

   Jason S. Lampkowski, Caitlin E. Durham, Marshall S. Padilla, and Douglas D. Young

   Organic & Biomolecular Chemistry, 2014

   @article{lampkowski2014polyynes,
     article_type = {Research Article},
     title = {Preparation of asymmetrical polyynes by a solid-supported Glaser-Hay reaction},
     author = {Lampkowski, Jason S. and Durham, Caitlin E. and Padilla, Marshall S. and Young, Douglas D.},
     journal = {Organic \& Biomolecular Chemistry},
     volume = {13},
     pages = {424--427},
     year = {2014},
     doi = {10.1039/C4OB02196G}
   }